The Fight against the Influenza A Virus H1N1: Synthesis, Molecular Modeling, and Biological Evaluation of Benzofurazan Derivatives as Viral RNA Polymerase Inhibitors
Identifieur interne : 000761 ( Main/Exploration ); précédent : 000760; suivant : 000762The Fight against the Influenza A Virus H1N1: Synthesis, Molecular Modeling, and Biological Evaluation of Benzofurazan Derivatives as Viral RNA Polymerase Inhibitors
Auteurs : Mafalda Pagano [Italie] ; Daniele Castagnolo [Italie] ; Martina Bernardini [Italie] ; Anna Lucia Fallacara [Italie] ; Ilaria Laurenzana [Italie] ; Davide Deodato [Italie] ; Ulrich Kessler [Suisse] ; Beatrice Pilger [Suisse] ; Lilli Stergiou [Suisse] ; Stephan Strunze [Suisse] ; Cristina Tintori [Italie] ; Maurizio Botta [Italie, États-Unis]Source :
- ChemMedChem [ 1860-7179 ] ; 2014-01.
Abstract
The influenza RNA polymerase complex, which consists of the three subunits PA, PB1, and PB2, is a promising target for the development of new antiviral drugs. A large library of benzofurazan compounds was synthesized and assayed against influenza virus A/WSN/33 (H1N1). Most of the new derivatives were found to act by inhibiting the viral RNA polymerase complex through disruption of the complex formed between subunits PA and PB1. Docking studies were also performed to elucidate the binding mode of benzofurazans within the PB1 binding site in PA and to identify amino acids involved in their mechanism of action. The predicted binding pose is fully consistent with the biological data and lays the foundation for the rational development of more effective PA–PB1 inhibitors.
In the fight against influenza virus A/WSN/33 (H1N1), the PA–PB1 protein–protein interaction is emerging as a new drug target. To identify small molecules able to inhibit the viral RNA polymerase complex, the benzofurazan scaffold was explored by synthesizing a large library of derivatives. Some compounds showed high anti‐H1N1 activity and emerged as effective inhibitors of the PA–PB1 interaction, with IC50 values in the micromolar range.
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DOI: 10.1002/cmdc.201300378
Affiliations:
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De Gasperi 2</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">ChemMedChem</title><title level="j" type="alt">CHEMMEDCHEM</title><idno type="ISSN">1860-7179</idno><idno type="eISSN">1860-7187</idno><imprint><biblScope unit="vol">9</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="129">129</biblScope><biblScope unit="page" to="150">150</biblScope><biblScope unit="page-count">22</biblScope><publisher>WILEY‐VCH Verlag</publisher><pubPlace>Weinheim</pubPlace><date type="published" when="2014-01">2014-01</date></imprint><idno type="ISSN">1860-7179</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1860-7179</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The influenza RNA polymerase complex, which consists of the three subunits PA, PB1, and PB2, is a promising target for the development of new antiviral drugs. A large library of benzofurazan compounds was synthesized and assayed against influenza virus A/WSN/33 (H1N1). Most of the new derivatives were found to act by inhibiting the viral RNA polymerase complex through disruption of the complex formed between subunits PA and PB1. Docking studies were also performed to elucidate the binding mode of benzofurazans within the PB1 binding site in PA and to identify amino acids involved in their mechanism of action. The predicted binding pose is fully consistent with the biological data and lays the foundation for the rational development of more effective PA–PB1 inhibitors.</div><div type="abstract" xml:lang="en">In the fight against influenza virus A/WSN/33 (H1N1), the PA–PB1 protein–protein interaction is emerging as a new drug target. To identify small molecules able to inhibit the viral RNA polymerase complex, the benzofurazan scaffold was explored by synthesizing a large library of derivatives. Some compounds showed high anti‐H1N1 activity and emerged as effective inhibitors of the PA–PB1 interaction, with IC50 values in the micromolar range.</div></front></TEI><affiliations><list><country><li>Italie</li><li>Suisse</li><li>États-Unis</li></country><region><li>Latium</li><li>Pennsylvanie</li></region><settlement><li>Rome</li></settlement><orgName><li>Université de Rome « La Sapienza »</li></orgName></list><tree><country name="Italie"><noRegion><name sortKey="Pagano, Mafalda" sort="Pagano, Mafalda" uniqKey="Pagano M" first="Mafalda" last="Pagano">Mafalda Pagano</name></noRegion><name sortKey="Bernardini, Martina" sort="Bernardini, Martina" uniqKey="Bernardini M" first="Martina" last="Bernardini">Martina Bernardini</name><name sortKey="Botta, Maurizio" sort="Botta, Maurizio" uniqKey="Botta M" first="Maurizio" last="Botta">Maurizio Botta</name><name sortKey="Botta, Maurizio" sort="Botta, Maurizio" uniqKey="Botta M" first="Maurizio" last="Botta">Maurizio Botta</name><name sortKey="Castagnolo, Daniele" sort="Castagnolo, Daniele" uniqKey="Castagnolo D" first="Daniele" last="Castagnolo">Daniele Castagnolo</name><name sortKey="Deodato, Davide" sort="Deodato, Davide" uniqKey="Deodato D" first="Davide" last="Deodato">Davide Deodato</name><name sortKey="Fallacara, Anna Lucia" sort="Fallacara, Anna Lucia" uniqKey="Fallacara A" first="Anna Lucia" last="Fallacara">Anna Lucia Fallacara</name><name sortKey="Fallacara, Anna Lucia" sort="Fallacara, Anna Lucia" uniqKey="Fallacara A" first="Anna Lucia" last="Fallacara">Anna Lucia Fallacara</name><name sortKey="Laurenzana, Ilaria" sort="Laurenzana, Ilaria" uniqKey="Laurenzana I" first="Ilaria" last="Laurenzana">Ilaria Laurenzana</name><name sortKey="Tintori, Cristina" sort="Tintori, Cristina" uniqKey="Tintori C" first="Cristina" last="Tintori">Cristina Tintori</name></country><country name="Suisse"><noRegion><name sortKey="Kessler, Ulrich" sort="Kessler, Ulrich" uniqKey="Kessler U" first="Ulrich" last="Kessler">Ulrich Kessler</name></noRegion><name sortKey="Pilger, Beatrice" sort="Pilger, Beatrice" uniqKey="Pilger B" first="Beatrice" last="Pilger">Beatrice Pilger</name><name sortKey="Stergiou, Lilli" sort="Stergiou, Lilli" uniqKey="Stergiou L" first="Lilli" last="Stergiou">Lilli Stergiou</name><name sortKey="Strunze, Stephan" sort="Strunze, Stephan" uniqKey="Strunze S" first="Stephan" last="Strunze">Stephan Strunze</name></country><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Botta, Maurizio" sort="Botta, Maurizio" uniqKey="Botta M" first="Maurizio" last="Botta">Maurizio Botta</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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